MagXtract® 3200 Application Showcase: Automated viral RNA extraction and PCR setup using Omega Bio-tek Mag-Bind® Universal Pathogen 96 Kit Application Note
■In response to the COVID-19 pandemic, the demands for nucleic acid purification and laboratory automation have increased dramatically. The Mag-Bind® Universal Pathogen 96 Kit (M4029) was developed by Omega Bio-tek to provide an automated solution for the purification of viral nucleic acids with pathogenic DNA from a range of samples. In this application note, we provide the automated workflow for viral RNA extraction and PCR set up for detection of SARS-CoV-2 RNA using the Mag-Bind Universal Pathogen 96 Kit on Chroma ATE’s MagXtract 3200. The product specifications of Chroma ATE’s MagXtract 3200 system are described in Table 1 below. The application note also discusses the performance of this automated workflow in terms of sensitivity of viral detection using real-time RT-PCR.
●Conclusions
■Omega Bio-tek’s Mag-Bind Universal Pathogen 96 Kit in conjunction with Chroma ATE’s MagXtract 3200 for viral RNA extraction presents a solution to the increasing demand for timely high throughput viral RNA purifications by streamlining the workflow for extraction and qPCR setup. The Mag-Bind Universal Pathogen 96 Kit has been shown to yield viral RNA which can be detected even at a low viral load, making it useful for detecting viruses such as SARS-CoV-2 via RT-PCR. This workflow can potentially save 80% of the hands-on time and provide researchers and scientists to conduct clinical work in a safe, accurate, and efficient manner.
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M4029 、 M4029-00 、 M4029-01 、 9-49200002 、 A94-000135 、 A94-000130 、 A94-000131 |
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Application note & Design Guide |
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Please see the document for details |
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English Chinese Chinese and English Japanese |
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2023/7/18 |
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759 KB |
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