Lasers versus LEDs for Bioinstrumentation Laser Advantage Note No.2 - Spectral Brightness White Paper

2024-03-11
●A laser is a monochromatic source that can yield a clean fluorescence signal with very high signal-to- noise ratio. In contrast, the LED is a broadband source that requires the cost and complexity of extra filters, and presents significant cross-talk challenges when detecting multiple fluorochromes.
●Summary:
■Laser. With a laser, all the light is at a single wavelength. And, with many laser wavelengths to choose from (a) the laser can be matched to the peak absorption of the fluorochrome, maximizing signal, and (b) a low cost longpass filter will completely eliminate any laser scatter, enabling very high signal to noise.
■LED. With a LED, the output light is spread over a very broad spectrum with a long wavelength “tail.” This means reduces system efficiency, compared to a laser, and means that additional filters must be used so that scattered unwanted photons are separated from the signal and don’t reach the photodetector. This becomes even more complex where multiple fluorochromes are targeted.
■Result. Even with its higher component cost, a laser enables higher signal, higher S/N and reduced optical complexity. Together with other laser advantages, the end result is that the laser enables a superior instrument with lower true overall costs per measurement.

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2021/6/11

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