Lasers versus LEDs for Bioinstrumentation Laser Advantage Note No.2 - Spectral Brightness White Paper

PublishTime：2024-03-11

●A laser is a monochromatic source that can yield a clean fluorescence signal with very high signal-to- noise ratio. In contrast, the LED is a broadband source that requires the cost and complexity of extra filters, and presents significant cross-talk challenges when detecting multiple fluorochromes.
●Summary:
■Laser. With a laser, all the light is at a single wavelength. And, with many laser wavelengths to choose from (a) the laser can be matched to the peak absorption of the fluorochrome, maximizing signal, and (b) a low cost longpass filter will completely eliminate any laser scatter, enabling very high signal to noise.
■LED. With a LED, the output light is spread over a very broad spectrum with a long wavelength “tail.” This means reduces system efficiency, compared to a laser, and means that additional filters must be used so that scattered unwanted photons are separated from the signal and don’t reach the photodetector. This becomes even more complex where multiple fluorochromes are targeted.
■Result. Even with its higher component cost, a laser enables higher signal, higher S/N and reduced optical complexity. Together with other laser advantages, the end result is that the laser enables a superior instrument with lower true overall costs per measurement.

Brand of Product：	COHERENT
Part#：	OBIS 355 LG 、 OBIS 375 LX 、 OBIS 405 LX 、 OBIS 422LX 、 OBIS 445 LX 、 OBIS 458 LX 、 OBIS 473LX 、 OBIS 488 LS 、 OBIS 488 LX 、 OBIS 505 LX 、 OBIS 514 LS 、 OBIS 514 LX 、 OBIS 520 LX 、 OBIS 532 LS 、 OBIS 552 LS 、 OBIS 561 LS 、 OBIS 637 LX 、 OBIS 640 LX 、 OBIS 647 LX 、 OBIS 660 LX 、 OBIS 685 LX 、 OBIS 730 LX 、 OBIS 785 LX More Part#
Product Category：	Lasers More
Application：	[ Bioinstrumentation ] More
Data Type：	White Paper More
Safety & Environment Standards：	More
Operating Temperature：	Please see the document for details
Qualification Grade：	More
Qualification Approval：	More
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Language：	English Chinese Chinese and English Japanese
Date of Creation：	2021/6/11
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Data Size：	589 KB

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