Technology for Real-Time Detection of Microbes in Water
●Introduction
▲Water is widely used in the production of cosmetics, foodstuffs, and pharmaceutical and other products as an ingredient, for washing, and for other purposes. Quality requirements for water put to such uses include the proper maintenance of equipment in order to keep the number of microbes in the water below a prescribed level, and the periodic monitoring of the number of microbes.
▲The size of a microbe varies with the species, but generally speaking microbes look like particles with a diameter of around 1 μm (Figure 1). Identifying them requires a method such as observation with a high-power microscope. However, since what water quality standards actually require is a low concentration of microbes, such as 0.1 CFU/ml (colony-forming units per ml) for water used in injections, and 100 CFU/ml for purified water, monitoring microbes with a microscope is not practical. As an alternative, therefore, culturing is widely employed for detecting microbes. Water potentially containing microbes is applied to a growth medium to encourage the formation of colonies, clusters of microbes that are visible after several days of growth. The number of colonies is counted visually or by image identification to estimate the number of microbes contained in the water.
▲The time-tested culturing method has high specificity and other advantages. Unfortunately, however, since this kind of test takes several days to determine the number of microbes, if equipment for producing purified water is contaminated, for example, there are risks associated with the fact that several days will pass before the contamination is detected and can be mitigated. Moreover, since some microbes do not readily form colonies, there is a risk that the contamination will not be detected. In addition, culturing raises operating costs because each periodic inspection incurs personnel costs for water sampling, culturing, counting, and related tasks.
▲For the foregoing reasons, there is a compelling need for a new technology that can detect water bioburden more quickly.
▲In response to this need, the IMD-W™ Online Water Bioburden Analyzer was developed. This article describes the measurement principle and design concept of the IMD-W, and the results of evaluating its basic performance.
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Supplier and Product Introduction |
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Please see the document for details |
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English Chinese Chinese and English Japanese |
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2015/6/19 |
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1.8 MB |
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