High-throughput Cell Function Discovery System CV7000 Spheroid Analysis Application Note

2021-11-27
◆Introduction
■The structures and functions of cells produced through spheroid formation are known to be more similar to live bodies than monolayer 2D organisms. Therefore, various formation methods and data accumulation for spheroids is progressing. We imaged spheroids using CellVoyager CV7000, and analyzed the images. Specifically, multiple images were captured along the Z-axis, reorganized into 2D image data, and evaluated with regard to the maximum spheroid diameter and number of contained cells. In this way, combination of the CV7000 high-speed 3D imaging and projection functions facilitates the efficient analysis of 3D samples. Furthermore, the method can be applied to live cells to establish new experimental methods such as the time series evaluation of cells.

YOKOGAWA

CV7000

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2021/05/26

AN80H01C05-01E 2nd

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