Confocal Quantitative Imaging Cytometer CQ1 Application Note
●Introduction
■Autophagy is the mechanism of the cell that disassembles unnecessary proteins and organelles to recycle or metabolize them. When placed in certain physiological conditions, the cells produce phagophore to cover unnecessary components and form autophagosome. The autophagosomefuses with lysosome (production of autolysosome), and acid hydrolase from lysosome degrades the unnecessary components. Autophagy has been confirmed to be related to cancer and neurodegenerative diseases such as Alzheimer and Parkinson’s disease, and as possessing a physiological function in aging.
■The following are the results of experiments using the CQ1 for imaging and the high content analysis software CellPathfinderfor analysis. In this experiment, DALGreen-Autophagy Detection (Donjindo Molecular Technologies, Inc.)*1was used; it penetrates cell membranes and is drawn into autophagosome along with unnecessary components, fusing with lysosome and then increasing fluorescence in the acid environment in autolysosome.
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Application note & Design Guide |
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Please see the document for details |
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English Chinese Chinese and English Japanese |
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2018/12/19 |
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AN 80J01A10-01E |
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712 KB |
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