Introduction

The Agilent7890B GC is the gold-standard instrument for the analysis of semivolatile

organic compounds (SVOCs) in high-throughput contract environmental laboratories

throughout the world. The performance of the 7890B GC has set the standard for the

analysis of SVOCs.

The AgilentIntuvo 9000 GC delivers the same high-quality performance as a 7890B

GC while providing advantages only attainable with the Intuvo 9000 GC including:

• Fast cooldown to improve throughput

• Easy column maintenance with clip-free columns

• Protection of the column from matrix fouling with the Guard Chip

This Application Brief demonstrates the equivalency in retention time and analyte

response to the 7890B GC using the same instrumental conditions without the need

for method translation.

Instrument Methods

To demonstrate equivalency, the same instrument conﬁguration and parameters were

used between the 7890B and the Intuvo 9000.

Sample

A mixture of 77 acids, bases, and neutrals at 20 µg/mL, and six internal standards at

40 µg/mL in dichloromethane was used.

RESPONSE AND RETENTION TIME

EQUIVALENCY TO AGILENT7890B GC

Technology Advantage: AgilentIntuvo 9000 GC with

Agilent5977 MSD

For more information, visit:

www.agilent.com/chem/intuvo

Information, descriptions and speciﬁcations in this

publication are subject to change without notice.

Published in USA, August 1, 2016

5991-7181EN

Results and Discussion

Figure 1 shows the normalized total ion chromatogram (TIC) for an injection of the

SVOC standard on the 7890B GC and on the Intuvo 9000 GC.

The chromatograms are virtually indistinguishable. The average difference in relative

retention time of the 77 target compounds is 0.0006 minutes, and the average

difference in response factors is 4.6 %.

If closely inspected, slight differences can be observed for the clusters of peaks

eluting at retention times around 12.5 and 16.5 minutes. These are attributed to small

differences in resolution as a result of normal variation in column performance, not

gas chromatograph performance.

Common 7890B and Intuvo 9000 GC/MS parameters

Parameter Value

Inlet Split/splitless 300 °C

Injection volume 1 µL

Pulsed splitless injection 60 psi to 0.5 minutes

Purge 50 mL/min at 0.5 minutes

Septum purge Switched ﬂow mode

3 mL/min

Column AgilentDB-5ms UI 30 m × 0.25 mm, 0.5µm

Constant column ﬂow 2 mL/min

Column temperature 40 °C for 2 minutes

20 °C/min to 260 °C

6 °C/min to 330 °C, hold 1.3 minutes

Agilent5977 MSD with inert ion source

Transfer line 330 °C

Source 330 °C

MS Quad 200 °C

Figure 1. Normalized TIC for the SVOC standard using an Agilent 7890B GC (A) and an Agilent Intuvo 9000 GC (B).

3.58.5 13.5

Time (min)

TIC Normalized response

18.5 23.5

3.5

Agilent 7890B GC

Agilent Intuvo 9000 GC

8.513.5

Time (min)

TIC Normalized response

18.5 23.5

Conclusion

The AgilentIntuvo 9000 GC provides

equivalent retention time and analyte

response using the same instrumental

conditions and method parameters

as the Agilent 7890B GC without the

need for method translation. For more

information on the analysis of SVOCs

using the Intuvo 9000 GC, refer to the

Application Note

Reference

1. Giardina, M. Analysis of

Semivolatile Organic Compounds

using the Intuvo9000 GC,

AgilentTechnologies Application

Note, publication number

5991-7180EN, 2016.